Journal: Journal of Applied Biological Chemistry

Article Title: Anti-inflammatory activity of Polyporus parvovarius mycelial culture filtrate

doi: 10.3839/jabc.2024.004

Figure Lengend Snippet: Fig. 1 HPLC chromatogram of Polyporus parvovarius (Pp) mycelium culture extract. The chromatograms were analyzed at a wavelength of 254 nm. Pp_DY, Pp cultured in DY; Pp_MY, Pp cultured in MY; Pp_MEB, Pp cultured in MEB; Pp_PDB, Pp cultured in PDB

Article Snippet: Primary antibody가 부착된 membrane을 각 항체에 해당하는 secondary antibody (Cell Signaling Technology)를 사용하여 실 온에서 60분간 동안 반응시키고 다시 0.05% T/TBS를 사용하 여 10분 간격으로 3번 세척을 반복한 뒤 Clarity Western ECL Substrate (1705061, Bio-Rad)와 반응시켜 Imaging densitometer (model GS-700, Bio-rad)를 통해 현상하였다.

Techniques: Cell Culture

Journal: Journal of Applied Biological Chemistry

Article Title: Anti-inflammatory activity of Polyporus parvovarius mycelial culture filtrate

doi: 10.3839/jabc.2024.004

Figure Lengend Snippet: Fig. 2 Studies on the anti-inflammatory activity of Pp culture filtrate. (A) Cytotoxicity of four types of media, (B) Nitric oxide production inhibitory activity of four types of media, (C) Cytotoxicity of Pp culture filtrate, (D) Inhibitory activity of nitric oxide production in Pp culture filtrate. Sample were treated with LPS-stimulated cells at concentrations of 50, 100, and 200 μg/mL. The data were presented as the means ± SD of data obtained three independent experiments. #p <0.05, ###p <0.001; compared to control, *p <0.05; **p <0.01; ***p <0.001; compared to LPS treated group

Article Snippet: Primary antibody가 부착된 membrane을 각 항체에 해당하는 secondary antibody (Cell Signaling Technology)를 사용하여 실 온에서 60분간 동안 반응시키고 다시 0.05% T/TBS를 사용하 여 10분 간격으로 3번 세척을 반복한 뒤 Clarity Western ECL Substrate (1705061, Bio-Rad)와 반응시켜 Imaging densitometer (model GS-700, Bio-rad)를 통해 현상하였다.

Techniques: Activity Assay, Control

Journal: Journal of Applied Biological Chemistry

Article Title: Anti-inflammatory activity of Polyporus parvovarius mycelial culture filtrate

doi: 10.3839/jabc.2024.004

Figure Lengend Snippet: Fig. 3 Inhibitory effects of Pp_DY on the generation of PGE2 in LPS- stimulated RAW264.7 cells. The generation of PGE2 was measured in the culture medium of cells stimulated with LPS (1 μg/mL) for 24 h in the presence of Pp_DY (50, 100, 200 μg/mL). The data were presented as the means ± SD of data obtained three independent experiments. ##p <0.01, compared to control, *p <0.05; **p <0.01, compared to LPS treated group

Article Snippet: Primary antibody가 부착된 membrane을 각 항체에 해당하는 secondary antibody (Cell Signaling Technology)를 사용하여 실 온에서 60분간 동안 반응시키고 다시 0.05% T/TBS를 사용하 여 10분 간격으로 3번 세척을 반복한 뒤 Clarity Western ECL Substrate (1705061, Bio-Rad)와 반응시켜 Imaging densitometer (model GS-700, Bio-rad)를 통해 현상하였다.

Techniques: Control

Journal: Journal of Applied Biological Chemistry

Article Title: Anti-inflammatory activity of Polyporus parvovarius mycelial culture filtrate

doi: 10.3839/jabc.2024.004

Figure Lengend Snippet: Fig. 4 Inhibitory effects of Pp_DY on the (A) iNOS, (B) COX-2 protein expression in LPS-stimulated RAW 264.7 cells. Cells (5×105 cells/well) were pre-incubated for 24 h, and then treated with LPS (1 μg/mL) and Pp_DY (50, 100, 200 μg/mL) for 24 h. β-actin was used as the control. The data were presented as the means ± SD of data obtained three independent experiments. ###p <0.001; compared to control, *p <0.05; **p <0.01; ***p <0.001, compared to LPS treated group

Article Snippet: Primary antibody가 부착된 membrane을 각 항체에 해당하는 secondary antibody (Cell Signaling Technology)를 사용하여 실 온에서 60분간 동안 반응시키고 다시 0.05% T/TBS를 사용하 여 10분 간격으로 3번 세척을 반복한 뒤 Clarity Western ECL Substrate (1705061, Bio-Rad)와 반응시켜 Imaging densitometer (model GS-700, Bio-rad)를 통해 현상하였다.

Techniques: Expressing, Incubation, Control

Journal: Journal of Applied Biological Chemistry

Article Title: Anti-inflammatory activity of Polyporus parvovarius mycelial culture filtrate

doi: 10.3839/jabc.2024.004

Figure Lengend Snippet: Fig. 5 Inhibitory effects of Pp_DY on the generation of pro-inflammatory cytokines in LPS-stimulated RAW264.7 cells. The generation of (A) TNF-α (B) IL-1β (C) IL-6 was measured by ELISA in the culture medium of cells stimulated with LPS (1 μg/mL) for 24 h in the presence of Pp_DY (50, 100, 200 μg/mL). The data were presented as the means ± SD of data obtained three independent experiments. ###p < 0.001, compared to control, *p <0.05; **p <0.01, compared to LPS treated group

Article Snippet: Primary antibody가 부착된 membrane을 각 항체에 해당하는 secondary antibody (Cell Signaling Technology)를 사용하여 실 온에서 60분간 동안 반응시키고 다시 0.05% T/TBS를 사용하 여 10분 간격으로 3번 세척을 반복한 뒤 Clarity Western ECL Substrate (1705061, Bio-Rad)와 반응시켜 Imaging densitometer (model GS-700, Bio-rad)를 통해 현상하였다.

Techniques: Enzyme-linked Immunosorbent Assay, Control